mouse anti cd44v6 Search Results


cd44v6 9a4  (Bio-Rad)
93
Bio-Rad cd44v6 9a4
a. ELISA for latent and active TGFβ1 in culture supernatants on day 4 (ILC1 from N=3 mice). b. Expression of Tgfb1 in primary murine ILC1 before (d0, N=4 mice) or after co-culture (d4, co-cultures derived from N=5 mice), with or without 2h PMA/Ionomycin activation (stim.). c. Expression of Tgfb1 in IEC from SIO only or SIO+ILC1 on d4, (from N=3 mice, x-axis scaled to 2b). d. Localization of TGFβR1 staining in SIO co-cultured with ILC1 (d4) counterstained for β-catenin (Rep. of N=3). e. RT-qPCR with exon-specific primers for CD44 splice variants s, v4, and v6 (ILC1 derived from N=3 mice). f . Representative confocal image of <t>CD44v6</t> localization in d4 SIO+ILC1, counterstained with F-Actin (Rep of N=3 mice). g. IPA of activated canonical pathways. Linked boxes contain overlapping differentially expressed genes (p adj beneath boxes; magenta-midnight colouring represents high-low z-score). h. Expression patterns of CD44v6, phosphorylated p38, β-catenin and DAPI nuclei in SIO alone, with ILC1, or with ILC1 and 1ng/ml TGFβ1,2,3-neutralization (Rep of N=3 mice, scale bars 50µm). i. Quantification of phosphorylated-p38 in the DAPI + region of the experiment in (h) (experiments from N=3 mice, each dot represents one nucleus) j. Quantification of β-catenin accumulation in IEC of experiment in (h), normalized to DAPI intensity (N=3 mice, each dot represents one cell). k. Cd44v6 expression and l. Axin2 expression after 2day culture of SIO with TGFβ1, with TGFβ1 and Pirfenidone (5µm) or TGFβ1 and PD16 (3µm) in N=3 three separate experiments. m. Quantification of crypt budding after 4 day co-culture (OneWay ANOVA and Tukey test, each dot one SIO, quantified from three experiments with ILC1 derived from N=3 mice). a,b,c,e,k,l Two-tailed unpaired t-test, error bars show S.E.M.; i,j,m, OneWay ANOVA and Tukey test, error bars S.E.M. Scale bars indicated in overlays.
Cd44v6 9a4, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd44v6 9a4/product/Bio-Rad
Average 93 stars, based on 1 article reviews
cd44v6 9a4 - by Bioz Stars, 2026-02
93/100 stars
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iv mouse anti human cd44v6  (Bio-Rad)
93
Bio-Rad iv mouse anti human cd44v6
a. ELISA for latent and active TGFβ1 in culture supernatants on day 4 (ILC1 from N=3 mice). b. Expression of Tgfb1 in primary murine ILC1 before (d0, N=4 mice) or after co-culture (d4, co-cultures derived from N=5 mice), with or without 2h PMA/Ionomycin activation (stim.). c. Expression of Tgfb1 in IEC from SIO only or SIO+ILC1 on d4, (from N=3 mice, x-axis scaled to 2b). d. Localization of TGFβR1 staining in SIO co-cultured with ILC1 (d4) counterstained for β-catenin (Rep. of N=3). e. RT-qPCR with exon-specific primers for CD44 splice variants s, v4, and v6 (ILC1 derived from N=3 mice). f . Representative confocal image of <t>CD44v6</t> localization in d4 SIO+ILC1, counterstained with F-Actin (Rep of N=3 mice). g. IPA of activated canonical pathways. Linked boxes contain overlapping differentially expressed genes (p adj beneath boxes; magenta-midnight colouring represents high-low z-score). h. Expression patterns of CD44v6, phosphorylated p38, β-catenin and DAPI nuclei in SIO alone, with ILC1, or with ILC1 and 1ng/ml TGFβ1,2,3-neutralization (Rep of N=3 mice, scale bars 50µm). i. Quantification of phosphorylated-p38 in the DAPI + region of the experiment in (h) (experiments from N=3 mice, each dot represents one nucleus) j. Quantification of β-catenin accumulation in IEC of experiment in (h), normalized to DAPI intensity (N=3 mice, each dot represents one cell). k. Cd44v6 expression and l. Axin2 expression after 2day culture of SIO with TGFβ1, with TGFβ1 and Pirfenidone (5µm) or TGFβ1 and PD16 (3µm) in N=3 three separate experiments. m. Quantification of crypt budding after 4 day co-culture (OneWay ANOVA and Tukey test, each dot one SIO, quantified from three experiments with ILC1 derived from N=3 mice). a,b,c,e,k,l Two-tailed unpaired t-test, error bars show S.E.M.; i,j,m, OneWay ANOVA and Tukey test, error bars S.E.M. Scale bars indicated in overlays.
Iv Mouse Anti Human Cd44v6, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iv mouse anti human cd44v6/product/Bio-Rad
Average 93 stars, based on 1 article reviews
iv mouse anti human cd44v6 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
fitc-labeled mouse anti-human cd44v6 monoclonal antibody  (Jingmei Biotech Co Ltd)
90
Jingmei Biotech Co Ltd fitc-labeled mouse anti-human cd44v6 monoclonal antibody
a. ELISA for latent and active TGFβ1 in culture supernatants on day 4 (ILC1 from N=3 mice). b. Expression of Tgfb1 in primary murine ILC1 before (d0, N=4 mice) or after co-culture (d4, co-cultures derived from N=5 mice), with or without 2h PMA/Ionomycin activation (stim.). c. Expression of Tgfb1 in IEC from SIO only or SIO+ILC1 on d4, (from N=3 mice, x-axis scaled to 2b). d. Localization of TGFβR1 staining in SIO co-cultured with ILC1 (d4) counterstained for β-catenin (Rep. of N=3). e. RT-qPCR with exon-specific primers for CD44 splice variants s, v4, and v6 (ILC1 derived from N=3 mice). f . Representative confocal image of <t>CD44v6</t> localization in d4 SIO+ILC1, counterstained with F-Actin (Rep of N=3 mice). g. IPA of activated canonical pathways. Linked boxes contain overlapping differentially expressed genes (p adj beneath boxes; magenta-midnight colouring represents high-low z-score). h. Expression patterns of CD44v6, phosphorylated p38, β-catenin and DAPI nuclei in SIO alone, with ILC1, or with ILC1 and 1ng/ml TGFβ1,2,3-neutralization (Rep of N=3 mice, scale bars 50µm). i. Quantification of phosphorylated-p38 in the DAPI + region of the experiment in (h) (experiments from N=3 mice, each dot represents one nucleus) j. Quantification of β-catenin accumulation in IEC of experiment in (h), normalized to DAPI intensity (N=3 mice, each dot represents one cell). k. Cd44v6 expression and l. Axin2 expression after 2day culture of SIO with TGFβ1, with TGFβ1 and Pirfenidone (5µm) or TGFβ1 and PD16 (3µm) in N=3 three separate experiments. m. Quantification of crypt budding after 4 day co-culture (OneWay ANOVA and Tukey test, each dot one SIO, quantified from three experiments with ILC1 derived from N=3 mice). a,b,c,e,k,l Two-tailed unpaired t-test, error bars show S.E.M.; i,j,m, OneWay ANOVA and Tukey test, error bars S.E.M. Scale bars indicated in overlays.
Fitc Labeled Mouse Anti Human Cd44v6 Monoclonal Antibody, supplied by Jingmei Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc-labeled mouse anti-human cd44v6 monoclonal antibody/product/Jingmei Biotech Co Ltd
Average 90 stars, based on 1 article reviews
fitc-labeled mouse anti-human cd44v6 monoclonal antibody - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
mouse anti-human cd44v6 gtx123973  (GeneTex)
90
GeneTex mouse anti-human cd44v6 gtx123973
a. ELISA for latent and active TGFβ1 in culture supernatants on day 4 (ILC1 from N=3 mice). b. Expression of Tgfb1 in primary murine ILC1 before (d0, N=4 mice) or after co-culture (d4, co-cultures derived from N=5 mice), with or without 2h PMA/Ionomycin activation (stim.). c. Expression of Tgfb1 in IEC from SIO only or SIO+ILC1 on d4, (from N=3 mice, x-axis scaled to 2b). d. Localization of TGFβR1 staining in SIO co-cultured with ILC1 (d4) counterstained for β-catenin (Rep. of N=3). e. RT-qPCR with exon-specific primers for CD44 splice variants s, v4, and v6 (ILC1 derived from N=3 mice). f . Representative confocal image of <t>CD44v6</t> localization in d4 SIO+ILC1, counterstained with F-Actin (Rep of N=3 mice). g. IPA of activated canonical pathways. Linked boxes contain overlapping differentially expressed genes (p adj beneath boxes; magenta-midnight colouring represents high-low z-score). h. Expression patterns of CD44v6, phosphorylated p38, β-catenin and DAPI nuclei in SIO alone, with ILC1, or with ILC1 and 1ng/ml TGFβ1,2,3-neutralization (Rep of N=3 mice, scale bars 50µm). i. Quantification of phosphorylated-p38 in the DAPI + region of the experiment in (h) (experiments from N=3 mice, each dot represents one nucleus) j. Quantification of β-catenin accumulation in IEC of experiment in (h), normalized to DAPI intensity (N=3 mice, each dot represents one cell). k. Cd44v6 expression and l. Axin2 expression after 2day culture of SIO with TGFβ1, with TGFβ1 and Pirfenidone (5µm) or TGFβ1 and PD16 (3µm) in N=3 three separate experiments. m. Quantification of crypt budding after 4 day co-culture (OneWay ANOVA and Tukey test, each dot one SIO, quantified from three experiments with ILC1 derived from N=3 mice). a,b,c,e,k,l Two-tailed unpaired t-test, error bars show S.E.M.; i,j,m, OneWay ANOVA and Tukey test, error bars S.E.M. Scale bars indicated in overlays.
Mouse Anti Human Cd44v6 Gtx123973, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-human cd44v6 gtx123973/product/GeneTex
Average 90 stars, based on 1 article reviews
mouse anti-human cd44v6 gtx123973 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


a. ELISA for latent and active TGFβ1 in culture supernatants on day 4 (ILC1 from N=3 mice). b. Expression of Tgfb1 in primary murine ILC1 before (d0, N=4 mice) or after co-culture (d4, co-cultures derived from N=5 mice), with or without 2h PMA/Ionomycin activation (stim.). c. Expression of Tgfb1 in IEC from SIO only or SIO+ILC1 on d4, (from N=3 mice, x-axis scaled to 2b). d. Localization of TGFβR1 staining in SIO co-cultured with ILC1 (d4) counterstained for β-catenin (Rep. of N=3). e. RT-qPCR with exon-specific primers for CD44 splice variants s, v4, and v6 (ILC1 derived from N=3 mice). f . Representative confocal image of CD44v6 localization in d4 SIO+ILC1, counterstained with F-Actin (Rep of N=3 mice). g. IPA of activated canonical pathways. Linked boxes contain overlapping differentially expressed genes (p adj beneath boxes; magenta-midnight colouring represents high-low z-score). h. Expression patterns of CD44v6, phosphorylated p38, β-catenin and DAPI nuclei in SIO alone, with ILC1, or with ILC1 and 1ng/ml TGFβ1,2,3-neutralization (Rep of N=3 mice, scale bars 50µm). i. Quantification of phosphorylated-p38 in the DAPI + region of the experiment in (h) (experiments from N=3 mice, each dot represents one nucleus) j. Quantification of β-catenin accumulation in IEC of experiment in (h), normalized to DAPI intensity (N=3 mice, each dot represents one cell). k. Cd44v6 expression and l. Axin2 expression after 2day culture of SIO with TGFβ1, with TGFβ1 and Pirfenidone (5µm) or TGFβ1 and PD16 (3µm) in N=3 three separate experiments. m. Quantification of crypt budding after 4 day co-culture (OneWay ANOVA and Tukey test, each dot one SIO, quantified from three experiments with ILC1 derived from N=3 mice). a,b,c,e,k,l Two-tailed unpaired t-test, error bars show S.E.M.; i,j,m, OneWay ANOVA and Tukey test, error bars S.E.M. Scale bars indicated in overlays.

Journal: Nature materials

Article Title: ILC1 drive intestinal epithelial and matrix remodelling

doi: 10.1038/s41563-020-0783-8

Figure Lengend Snippet: a. ELISA for latent and active TGFβ1 in culture supernatants on day 4 (ILC1 from N=3 mice). b. Expression of Tgfb1 in primary murine ILC1 before (d0, N=4 mice) or after co-culture (d4, co-cultures derived from N=5 mice), with or without 2h PMA/Ionomycin activation (stim.). c. Expression of Tgfb1 in IEC from SIO only or SIO+ILC1 on d4, (from N=3 mice, x-axis scaled to 2b). d. Localization of TGFβR1 staining in SIO co-cultured with ILC1 (d4) counterstained for β-catenin (Rep. of N=3). e. RT-qPCR with exon-specific primers for CD44 splice variants s, v4, and v6 (ILC1 derived from N=3 mice). f . Representative confocal image of CD44v6 localization in d4 SIO+ILC1, counterstained with F-Actin (Rep of N=3 mice). g. IPA of activated canonical pathways. Linked boxes contain overlapping differentially expressed genes (p adj beneath boxes; magenta-midnight colouring represents high-low z-score). h. Expression patterns of CD44v6, phosphorylated p38, β-catenin and DAPI nuclei in SIO alone, with ILC1, or with ILC1 and 1ng/ml TGFβ1,2,3-neutralization (Rep of N=3 mice, scale bars 50µm). i. Quantification of phosphorylated-p38 in the DAPI + region of the experiment in (h) (experiments from N=3 mice, each dot represents one nucleus) j. Quantification of β-catenin accumulation in IEC of experiment in (h), normalized to DAPI intensity (N=3 mice, each dot represents one cell). k. Cd44v6 expression and l. Axin2 expression after 2day culture of SIO with TGFβ1, with TGFβ1 and Pirfenidone (5µm) or TGFβ1 and PD16 (3µm) in N=3 three separate experiments. m. Quantification of crypt budding after 4 day co-culture (OneWay ANOVA and Tukey test, each dot one SIO, quantified from three experiments with ILC1 derived from N=3 mice). a,b,c,e,k,l Two-tailed unpaired t-test, error bars show S.E.M.; i,j,m, OneWay ANOVA and Tukey test, error bars S.E.M. Scale bars indicated in overlays.

Article Snippet: CD44v6 (9A4) , Rat IgG1 , BioRad , MCA1967 , Mouse , 1:500.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Co-Culture Assay, Derivative Assay, Activation Assay, Staining, Cell Culture, Quantitative RT-PCR, Neutralization, Two Tailed Test

a. Expression of IFNG and IL22 in biopsy-derived hILC1 after 7 day co-culture with HIO with (ILC1 co-cultures from N=3 patients) or without (ILC1 co-cultures from N=6 patients) 2h PMA/Ionomycin stimulation (stim.), with corresponding represenative images of E-cadherin, CD44v6, and CD45 staining of HIO cultures with or without inflamed hILC1 shown in b (Scale 50µm), white box indicates magnified crypt (Scale 20µm). c. Expression of CD44s and CD44v6 in FACS-purified IEC from HIO only, hILC1 co-culture, from inflamed (inf.) or uninflamed (un.) samples (N=3 patients per condition). d. Proportion of hILC1 relative to other Lineage - CD127 + ILC subtypes prior to co-culture (ILC from N=4 uninflamed and N=4 inflamed tissue biopsies). e. Log 10 cell count of hILC1 before (d0, from N=4 patients) and after (d7, from N=3 patients) co-culture from un. and inf. biopsies. f. Relative expression of TGFB1 in hILC1 from N=3 inf., N=3 un., or N=3 stim. different patients after 7 day co-culture with HIO. g. Immunohistochemistry from biopsies from IBD patients with (right) or without (left) active inflammation. CD45 lymphocytes also express E-cadherin and CD44v6. h. SMA+ myofibroblast and VIMENTIN+ fibroblast organization around the epithelium in an inflamed patient biopsy and in HIO+ILC1 co-cultures. i. Relative expression of CD44v6 in EpCAM - CD45 - CD90 + fibroblasts (FB) purified from HIO after 7 day culture with or without inflamed hILC1 (ILC1 from N=3 inflamed tissue biopsies). a,c,e,f OneWay ANOVA with Tukey’s test, error bars S.E.M; d,i, unpaired two-tailed t-test, error bars S.E.M.. Scale bars 50µm.

Journal: Nature materials

Article Title: ILC1 drive intestinal epithelial and matrix remodelling

doi: 10.1038/s41563-020-0783-8

Figure Lengend Snippet: a. Expression of IFNG and IL22 in biopsy-derived hILC1 after 7 day co-culture with HIO with (ILC1 co-cultures from N=3 patients) or without (ILC1 co-cultures from N=6 patients) 2h PMA/Ionomycin stimulation (stim.), with corresponding represenative images of E-cadherin, CD44v6, and CD45 staining of HIO cultures with or without inflamed hILC1 shown in b (Scale 50µm), white box indicates magnified crypt (Scale 20µm). c. Expression of CD44s and CD44v6 in FACS-purified IEC from HIO only, hILC1 co-culture, from inflamed (inf.) or uninflamed (un.) samples (N=3 patients per condition). d. Proportion of hILC1 relative to other Lineage - CD127 + ILC subtypes prior to co-culture (ILC from N=4 uninflamed and N=4 inflamed tissue biopsies). e. Log 10 cell count of hILC1 before (d0, from N=4 patients) and after (d7, from N=3 patients) co-culture from un. and inf. biopsies. f. Relative expression of TGFB1 in hILC1 from N=3 inf., N=3 un., or N=3 stim. different patients after 7 day co-culture with HIO. g. Immunohistochemistry from biopsies from IBD patients with (right) or without (left) active inflammation. CD45 lymphocytes also express E-cadherin and CD44v6. h. SMA+ myofibroblast and VIMENTIN+ fibroblast organization around the epithelium in an inflamed patient biopsy and in HIO+ILC1 co-cultures. i. Relative expression of CD44v6 in EpCAM - CD45 - CD90 + fibroblasts (FB) purified from HIO after 7 day culture with or without inflamed hILC1 (ILC1 from N=3 inflamed tissue biopsies). a,c,e,f OneWay ANOVA with Tukey’s test, error bars S.E.M; d,i, unpaired two-tailed t-test, error bars S.E.M.. Scale bars 50µm.

Article Snippet: CD44v6 (9A4) , Rat IgG1 , BioRad , MCA1967 , Mouse , 1:500.

Techniques: Expressing, Derivative Assay, Co-Culture Assay, Staining, Purification, Cell Counting, Immunohistochemistry, Two Tailed Test

a. Murine ILC1 drive epithelial crypt budding in small intestine organoids through TGFβ1-induced phosphorylation of p38γ, which drives β-catenin accumulation and expression of Axin2 and CD44v6 . We propose that CD44v6 and β-catenin might engage in a positive feedback loop, driving epithelial subtype-non-specific proliferation. b. hILC1 derived from IBD patients express TGFB1 and MMP9. ILC1 isolated from tissues with active inflammation also drive expression of both epithelial and mesenchymal CD44v6 in HIO. Moreover, these patient-derived ILC1 drive increased deposition of Fibronectin1 and MMP-mediated matrix degradation, resulting in a balance of matrix softening and stiffening. c. IPA of the murine RNA-sequencing dataset showing cumulative gene enrichment (z-score) in SIO co-cultured with ILC1 indicative of activation (magenta) and inhibition (blue) of selected gastrointestinal and inflammatory diseases and function (ILC1 derived from N=3 mice).

Journal: Nature materials

Article Title: ILC1 drive intestinal epithelial and matrix remodelling

doi: 10.1038/s41563-020-0783-8

Figure Lengend Snippet: a. Murine ILC1 drive epithelial crypt budding in small intestine organoids through TGFβ1-induced phosphorylation of p38γ, which drives β-catenin accumulation and expression of Axin2 and CD44v6 . We propose that CD44v6 and β-catenin might engage in a positive feedback loop, driving epithelial subtype-non-specific proliferation. b. hILC1 derived from IBD patients express TGFB1 and MMP9. ILC1 isolated from tissues with active inflammation also drive expression of both epithelial and mesenchymal CD44v6 in HIO. Moreover, these patient-derived ILC1 drive increased deposition of Fibronectin1 and MMP-mediated matrix degradation, resulting in a balance of matrix softening and stiffening. c. IPA of the murine RNA-sequencing dataset showing cumulative gene enrichment (z-score) in SIO co-cultured with ILC1 indicative of activation (magenta) and inhibition (blue) of selected gastrointestinal and inflammatory diseases and function (ILC1 derived from N=3 mice).

Article Snippet: CD44v6 (9A4) , Rat IgG1 , BioRad , MCA1967 , Mouse , 1:500.

Techniques: Phospho-proteomics, Expressing, Derivative Assay, Isolation, RNA Sequencing, Cell Culture, Activation Assay, Inhibition

Journal: Nature materials

Article Title: ILC1 drive intestinal epithelial and matrix remodelling

doi: 10.1038/s41563-020-0783-8

Figure Lengend Snippet:

Article Snippet: CD44v6 (9A4) , Rat IgG1 , BioRad , MCA1967 , Mouse , 1:500.

Techniques:

Journal: Nature materials

Article Title: ILC1 drive intestinal epithelial and matrix remodelling

doi: 10.1038/s41563-020-0783-8

Figure Lengend Snippet:

Article Snippet: CD44v6 (9A4) , Rat IgG1 , BioRad , MCA1967 , Mouse , 1:500.

Techniques:

Journal: Nature materials

Article Title: ILC1 drive intestinal epithelial and matrix remodelling

doi: 10.1038/s41563-020-0783-8

Figure Lengend Snippet:

Article Snippet: CD44v6 (9A4) , Rat IgG1 , BioRad , MCA1967 , Mouse , 1:500.

Techniques: